T4 dna ligase m0202l
WebT4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I ( E. coli ). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function. WebM0202L / Lot: 10183117 Page 1 of 3 Product Name: T4 DNA Ligase Catalog Number: M0202L Concentration: 400,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of 6 µg of Lambda-HindIII DNA in 30 minutes at 16°C in a total reaction volume of 20 µl. Packaging Lot Number: 10183117 Expiration Date ...
T4 dna ligase m0202l
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WebFor cohesive (sticky) ends, use 1 µl of T4 DNA Ligase in a 20 µl reaction for 10 minutes. For blunt ends, use 1 µl of T4 DNA Ligase in a 20 µl reaction for 2 hours or 1 µl high … WebProduct Name: T4 DNA Ligase Catalog Number: M0202L Concentration: 400,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of 6 µg of Lambda-HindIII DNA in 30 minutes at 16°C in a total reaction volume of 20 µl.
WebM0202L / Lot: 10083328 Page 1 of 3 Product Name: T4 DNA Ligase Catalog Number: M0202L Concentration: 400,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of 6 µg of Lambda-HindIII DNA in 30 minutes at 16°C in a total reaction volume of 20 µl. Packaging Lot Number: 10083328 Expiration Date ... WebJun 8, 2016 · Temperature optimum of the most commonly using T4 DNA ligase is around 37˚С. Therefore, the best choice for blunt-ended DNA fragments is 37˚С. However, sticky ends are often too short to form ...
WebProducts &. Applications. Cloning / Synthetic BiologyDNA Amplification / PCR & qPCRNext Generation SequencingCompetent CellsProteinexpressionLuciferase Reporter … WebM0202L / Lot: 10009351 Page 1 of 3 Product Name: T4 DNA Ligase Catalog Number: M0202L Concentration: 400,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of 6 µg of Lambda-HindIII DNA in 30 minutes at 16°C in a total reaction volume of 20 µl. Lot Number: 10009351 Expiration Date: 11/2024
WebM0202L / Lot: 10042433 Page 1 of 3 Product Name: T4 DNA Ligase Catalog Number: M0202L Concentration: 400,000 U/ml Unit Definition: One unit is defined as the amount …
http://sjgl.swu.edu.cn/lab/orders/product/38070041025137 emoninail fungal treatmentWebFor cohesive (sticky) ends, use 1 µl of T4 DNA Ligase in a 20 µl reaction for 10 minutes. For blunt ends, use 1 µl of T4 DNA Ligase in a 20 µl reaction for 2 hours or 1 µl high concentration T4 DNA Ligase for 10 minutes. Alternatively, NEB's Quick Ligation Kit (#M2200S) [30 reactions] or (#M2200L) [150 reactions]) is uniquely formulated to ... emo nite phillyWebT4 DNA Ligase, Article Number: m0202l. Products Login (0) Login . Cart 0. Products & Applications. Cloning / Synthetic Biology DNA Amplification / PCR & qPCR Next Generation Sequencing Competent Cells Proteinexpression Luciferase ... T4 DNA Ligase Packaging Unit: 100.000 units drake the wheelchair kidWebFirst, a phosphorylated DNA template's cyclization occurs (Fig.1) using T4 DNA ligase (M0202L) and a sequence specific primer (Note: this circular DNA is not our target). Next, we purified the circular product using Exonuclease I (M0293S), Exonuclease III (M0206S) and a PCR purification kit (740609.50). emo nite athens gaWebT4 DNA Ligase Vector DNA ( 4kb) Insert DNA ( 1kb) Nuclease-free water Procedure Set up the T4 DNA Ligase Reaction Note: T4 DNA Ligase should be added last. The table shows a ligation using a molar ratio of 1:3 vector to insert for the indicated DNA sizes. Use NEB calculator to calculate molar ratios. drake the weeknd liveWebT4 DNA Ligase will ligate these substrates: dsDNA Nicked DNA/RNA Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl … emo night west palm beachWebCRISPR/Cas9‐mediated KI methods rely on the DNA repair pathway, including the non‐homologous end joining (NHEJ) and homology‐directed repair (HDR) pathways when induced by double‐strand breaks (DSBs) at the target genomic sequence. ... Ipswich, MA) for USER cloning or Bbs1 (R0539L, NEB) and T4 DNA ligase (M0202L, NEB) for … drake this a roley not a stopwatch