Human myotubes eps mtor
Web8 dec. 2024 · Applying EPS to differentiated myotubes mimics motor neuron activation in muscle fibers and induces myotube contraction in vitro 13. EPS also has the metabolic properties of in vivo... Web23 sep. 2024 · At this point, myotubes were nutrient deprived in Earl's Balanced Salt Solution (EBSS, ThermoFisher Scientific) for ∼14 h, with a subset of myotubes maintained in DMEM (2%HS, 1%PS) to serve as a ‘baseline’ condition.
Human myotubes eps mtor
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Web3 nov. 2024 · EPS induced a decrease in the diameter, covered surface and expression of MHC1 in COPD myotubes. Although the expression of protein degradation markers was … Web15 apr. 2024 · Myotubes grown in 24-well plates (for palmitate oxidation measurement) or 6-well plates (for the other experiments) were transfected with 0.6 µg/well (24-well plates) or 2 µg/well (6-well plates) of pCMV3-ATF4 (Sino Biological Inc., Wayne, PA) or 50 mM siRNA (Santa Cruz Biotechnology Inc., Dallas, TX), using Lipofectamine 2000 (Invitrogen, …
WebAdherent human myotubes were either innervated with rat spinal cord segments or exposed to EPS. The expression pattern of MyHCs was assessed by quantitative polymerase … Web28 jul. 2024 · Human myotubes from four donors were exposed to electrical pulse stimulation (EPS) or control for 48 h after which gene expression was profiled. Contributor(s) Mengeste AM, Nikolic N, Fernandez AD, Feng YZ, Nyman TA, Kersten S, Haugen F, Kase ET, Aas V, Rustan AC, Thoresen GH Citation(s) 35874526 Submission date Apr 22, …
Web5 nov. 2024 · Search life-sciences literature (41,988,656 articles, preprints and more) Search Web20 jan. 2024 · Contractile activity of human myotubes with or without mouse fibroblast feeders. After 7–8 days of differentiation, EPS (1 Hz frequency, 4-ms duration, 20 V/25 …
Web25 jun. 2011 · Human myotubes were treated with leucine, insulin or co-treated with leucine and insulin for 30 min, 3 h or 24 h. Activation of mTOR signalling kinases were …
Web15 apr. 2024 · Methods: The human skeletal muscle cell line LHCN-M2 and mice exposed to different inducers of ER stress were used. Results: Palmitate- or tunicamycin-induced ER stress resulted in PGC-1α downregulation and enhanced expression of activating transcription factor 4 (ATF4) in human myotubes and mouse skeletal muscle. bouchon siggWeb31 aug. 2024 · Myoblasts obtained from human muscle biopsies ( n = 5) were differentiated into multinucleated myotubes and exposed to 8 h of EPS consisting of 2 ms pulses at … bouchon silicone stlbouchon siphonWeb22 mrt. 2012 · Electrical pulse stimulation (EPS) was applied to adherent human myotubes. Cellular contents of ATP, phosphocreatine (PCr) and lactate were determined. Glucose and oleic acid metabolism were studied using radio-labeled substrates, and gene expression was analyzed using real-time RT-PCR. bouchon silicone moldsWebThe present study revealed that EPS-evoked muscle contractile stimulation in 3D-EM tends to increase the concentration of lactate in the culture medium, which is consistent with previous studies... bouchon singaporeWeb20 feb. 2024 · The mammalian target of rapamycin (mTOR) complex exerts a pivotal role in protein anabolism and cell growth. Despite its importance, few studies adequately … bouchon siphon doucheWebskeletal muscle cells of human and animal origin. Motor neuron activation of muscle fibres can be replaced by applying EPS on differentiated skeletal muscle cells (myotubes) in culture [1,2]. Here we describe two protocols for EPS … bouchon shortbread recipe