WebOne-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling-circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined. WebMar 21, 2024 · Here we developed a droplet digital branched rolling circle amplification (ddBRCA) platform for the rapid and washing-free detection of cancer EVs. The design …
Electrical and Electrochemical Monitoring of Nucleic Acid Amplification …
WebLOC DEVICE FOR GENETIC ANALYSIS WHICH PERFORMS NUCLEIC ACID AMPLIFICATION BEFORE REMOVING NON-NUCLEIC ACID CONSTITUENTS IN A DIALYSIS SECTION: 申请号: US13149924: 申请日: 20 WebThe aptamer is linked to a primer sequence that is complementary to a G-quadruplex circular template. The presence of target exosomes could trigger rolling circle amplification and produce multiple G-quadruplex units. This horseradish peroxidase mimicking DNAzyme could catalyze the reduction of H 2 O 2 and generate … nsw heti learning
Rapid, sensitive and highly specific label-free fluorescence biosensor ...
WebFeb 15, 2024 · A simple fluorescence biosensor for rapid and sensitive target microRNA (miRNA) quantification by branched rolling circle amplification (BRCA) is developed in this work. Target miRNA functions as primer to recognize and hybridize with a circle DNA template, initiating rolling circle amplification (RCA) by Phi29 DNA polymerase. WebFeb 15, 2024 · Branched rolling circle amplification (BRCA) is the RCA derivative with an exponential amplification power. In BRCA, two kinds of primers are used in this type of amplification, one is the forward primer (target miRNA), and another is the reverse primer with a matching sequence to a formed single-strand DNA sequence by the forward … WebJan 1, 2024 · Detection of specific genes related to drug action can provide scientific guidance for personalized medicine. Taking the detection of a single-nucleotide … nsw heti courses