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Branched rolling circle amplification

WebOne-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling-circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined. WebMar 21, 2024 · Here we developed a droplet digital branched rolling circle amplification (ddBRCA) platform for the rapid and washing-free detection of cancer EVs. The design …

Electrical and Electrochemical Monitoring of Nucleic Acid Amplification …

WebLOC DEVICE FOR GENETIC ANALYSIS WHICH PERFORMS NUCLEIC ACID AMPLIFICATION BEFORE REMOVING NON-NUCLEIC ACID CONSTITUENTS IN A DIALYSIS SECTION: 申请号: US13149924: 申请日: 20 WebThe aptamer is linked to a primer sequence that is complementary to a G-quadruplex circular template. The presence of target exosomes could trigger rolling circle amplification and produce multiple G-quadruplex units. This horseradish peroxidase mimicking DNAzyme could catalyze the reduction of H 2 O 2 and generate … nsw heti learning https://benoo-energies.com

Rapid, sensitive and highly specific label-free fluorescence biosensor ...

WebFeb 15, 2024 · A simple fluorescence biosensor for rapid and sensitive target microRNA (miRNA) quantification by branched rolling circle amplification (BRCA) is developed in this work. Target miRNA functions as primer to recognize and hybridize with a circle DNA template, initiating rolling circle amplification (RCA) by Phi29 DNA polymerase. WebFeb 15, 2024 · Branched rolling circle amplification (BRCA) is the RCA derivative with an exponential amplification power. In BRCA, two kinds of primers are used in this type of amplification, one is the forward primer (target miRNA), and another is the reverse primer with a matching sequence to a formed single-strand DNA sequence by the forward … WebJan 1, 2024 · Detection of specific genes related to drug action can provide scientific guidance for personalized medicine. Taking the detection of a single-nucleotide … nsw heti courses

PNA Beacons for Duplex DNA - Antisense and Nucleic Acid Drug …

Category:A simple fluorescence aptasensor for gastric cancer …

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Branched rolling circle amplification

The Discovery of Rolling Circle Amplification and …

WebHere, we developed a fluorescence assay for the determination of gastric cancer exosomes based on branched rolling circle amplification (BRCA) and an aptamer to target … WebGENETIC ANALYSIS LOC FOR PCR AMPLIFICATION OF NUCLEIC ACIDS FROM WHOLE BLOOD: 申请号: US13150049: 申请日: 2011-06-01: 公开(公告)号: US20110312712A1: 公开(公告)日:

Branched rolling circle amplification

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WebLOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS, INCUBATION AND TANDEM NUCLEIC ACID AMPLIFICATION: 申请号: US13150027: 申请日: 2011-06-01: 公开(公告)号: U WebJan 4, 2024 · Here, we describe the development of a one-step branched rolling circle amplification (BRCA) method to measure serum c …

WebHere, we present a novel method for effective and sensitive detection of Chattonella marina using hyper-branched rolling circle amplification (HRCA) and HRCA-based strip test … WebAs of recent, the Saltatory Rolling Circle Amplification (SRCA) ... recognition ability of the molecular beacon towards the targeted bacteria DNA was integrated into an isothermal hyper-branched rolling circle amplification procedure during which a single molecule of the target bacteria DNA could be detected.

Web15 hours ago · A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification. Nanoscale, 12 (4) (2024), pp. 2445-2451. ... biosensing for BCR-ABL fusion gene detection combined with dual-toehold strand displacement reaction for signal amplification. Biosens. Bioelectron., 210 (2024), p. … WebRolling circle amplification (RCA) is an isothermal enzymatic process where a short DNA or RNA primer is amplified to form a long single stranded DNA or RNA using a circular …

WebLOC DEVICE WITH NUCLEIC ACID AMPLIFICATION SECTION: 申请号: US13150079: 申请日: 2011-06-01: 公开(公告)号: US20110312733A1: 公开(公告)日: 2011-12-22: 申请人: Meh

WebMay 15, 2011 · Target RNA primed rolling circle amplification (branched rolling circle amplification) Either in vitro transcribed target RNA or non-target RNA was mixed with … nike club sweatpantsWebHere we developed a droplet digital branched rolling circle amplification (ddBRCA) platform for the rapid and sensitive detection of cancer EVs. The design includes a hairpin structure DNA containing a cancer EVs specific aptamer sequence and BRCA primer sequence. The presence of target EVs could cause the conformational switching of the ... nsw highlightsWeb@article{Huang2024WashingFreeDO, title={Washing-Free Detection of Cancer Cell-Derived Extracellular Vesicles Based on Droplet Digital Branched Rolling Circle Amplification}, author={Rongrong Huang and Kaili Di and Khan Adeel and Boyue Fan and Xinrui Gu and Hongpan Xu and Han Shen and Nongyue He and Zhiyang Li}, journal={SSRN Electronic … nike club team sweatpantsWebMar 5, 2015 · Rolling circle amplification (RCA) ... For highly sensitive determination of small nucleic acids, branched RCA (BRCA) by introduction of a second primer complementary to the RCA product was developed (Lizardi et al., 1998; Cheng et al., 2009). BRCA can improve the reaction efficiency compared with conventional linear RCA by … nike club sweatpants anthraciteWebJan 1, 2024 · Here, we show that ramified rolling circle amplification (RCA) can be used to produce milligram amounts of a genomic nucleosomal DNA fragment in a scalable, one-pot reaction overnight. The protocol is efficient and flexible in choice of DNA sequence. ... branched, double-stranded repeat of the desired sequence. It should be noted that both ... nsw high countryWebGENETIC ANALYSIS LOC FOR NUCLEIC ACID AMPLIFICATION USING A NICKING ENZYME AND A DNA POLYMERASE: 申请号: US13150059: 申请日: 2011-06-01: 公开(公告)号: US20110312575A1: nike club tracksuit blueWebFeb 1, 2024 · Branched rolling circle amplification produces single-stranded amplicons that can aggregate detection probe-modified AuNPs, which induces a strong photoacoustic signal at 640 nm due to both the surface plasmon resonance shift and the size-dependent effect of laser-induced nanobubbles, achieving a sub-femtomolar detection limit within a … nike club team swoosh backpack